Download Analytical Methods in Human Toxicology: Part 2 by Alan S. Curry PDF

By Alan S. Curry

This selection of 9 essays by way of best experts considers the applying of the main lately built analytical ideas and gear to specific poisons and medicine. It describes using HPLC, fluorescence research, and radioimmunoassays in toxicology and exhibits equipment particular to forensic medication.

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Air dissolved in the mobile phase , particularly in water/solvent mixtures , may come out of solution and form troublesome air bubbles while under pressure during chromatography, so the 'degassing' of the mobile phase is an essential procedure prior to use. Degassing may be achieved either by placing the mobile phase in a vacuum, by helium purging, by immersion in an ultrasonic bath for a few minutes or by continuous , gentle magnetic stirring for an hour before and during use. 2 The Pump The pump is responsible for maintaining a flow of the mobile phase through the injector, column and detector.

3 BASIC CONCEPTS In order to evaluate, modify or improve a chromatographic system , some familiarity with the basic concepts of HPLC is required. These techniques include retention time , capacity facto r, resolution , selectivity factor and efficiency . The following sections describe simply the measurement and use of these basic parameters and illustrate their application to a typical toxicological analysis. 1 The Chromatogram The chromatogram provides a record of changes in the absorbance or fluorescence , etc.

E. linear and closely fitting the regression line). These standard curves should be spiked into the relevant matrix and carried through the entire analytical procedure . If sample preparation procedures are involved then the recovery of the compound of interest from the matrix should be compared to that of a 'non-processed' standard. The method should be checked for specificity. g. drugs used in combination with the compound of interest) should be checked for interference . If a blank of the sample matrix is available this should also be checked for interference.

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